OrangerV1, Main, Exploration, bibRecord, 001C59

Reduction in susceptibility to Xanthomonas axonopodis pv. citri in transgenic Citrus sinensis expressing the rice Xa21 gene

Identifieur interne : 001C59 ( Main/Exploration ); précédent : 001C58; suivant : 001C60

Reduction in susceptibility to Xanthomonas axonopodis pv. citri in transgenic Citrus sinensis expressing the rice Xa21 gene

Auteurs : B. M. J. Mendes [États-Unis] ; S. C. Cardoso [États-Unis] ; R. L. Boscariol-Camargo [États-Unis] ; R. B. Cruz [États-Unis] ; F. A. A. Mourao Filho [États-Unis] ; A. Bergamin Filho [Brésil]

Source :

Plant pathology [ 0032-0862 ] ; 2010.

RBID : Pascal:10-0069435

Descripteurs français

Pascal (Inist)
- Réduction, Sensibilité, Bactériose, Gène, Phytopathologie, Rutaceae, Xanthomonas axonopodis, Organisme génétiquement modifié, Citrus sinensis, Oryza, Agrume, Chancre plante.
Wicri :
- topic : Organisme génétiquement modifié, Agrume.

English descriptors

KwdEn :
- Bacteriosis, Citrus fruit, Citrus sinensis, Gene, Genetically modified organism, Oryza, Plant cankers, Plant pathology, Reduction, Rutaceae, Sensitivity, Xanthomonas axonopodis.

Abstract

This work reports the evaluation of citrus canker resistance in transgenic lines of four sweet orange cultivars Hamlin, Natal, Pera and Valencia expressing the rice Xa21 R-gene. The Agrobacterium-mediated genetic transformation was performed and transgene integration was confirmed by Southern blot analyses. These analyses showed that the plants had undergone 1-3 independent transgene integration events. The genetic transformation efficiency was 18.6% for Hamlin, 8.3% for Natal, 3% for Pera and 11.3% for Valencia sweet orange. Transgene transcription was confirmed by northern blot analyses in most transgenic lines analysed. Transgenic lines of each of the cultivars were selected and propagated for resistance evaluation to Xanthomonas axonopodis pv. citri. Transgenic lines with a higher resistance to citrus canker were identified for cvs Hamlin, Natal and Pera. The lowest diseased leaf area values were detected for transgenic lines of Natal and Pera, cultivars which are considered moderately resistant to citrus canker in the field, indicating an influence of cultivar genetic background on the effectiveness of the Xa21 gene in controlling the pathogen. Transgenic lines in which transgene transcription was not detected did not show any improvement in resistance to the pathogen. These results show the integration and expression of the rice Xa21 gene in citrus and the possibility of using R-genes in controlling diseases in a heterologous genetic background.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000272
to stream PascalFrancis, to step Curation: 000728
to stream PascalFrancis, to step Checkpoint: 000213
to stream Main, to step Merge: 001C97
to stream Main, to step Curation: 001C59

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Reduction in susceptibility to Xanthomonas axonopodis pv. citri in transgenic Citrus sinensis expressing the rice Xa21 gene</title>
<author><name sortKey="Mendes, B M J" sort="Mendes, B M J" uniqKey="Mendes B" first="B. M. J." last="Mendes">B. M. J. Mendes</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratório de Biotecnologia Vegetal, CENA/USP</s1>
<s2>13400-970 Piracicaba, SP</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>13400-970 Piracicaba, SP</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cardoso, S C" sort="Cardoso, S C" uniqKey="Cardoso S" first="S. C." last="Cardoso">S. C. Cardoso</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Dept. de Produção Vegetal, ESALQ/USP</s1>
<s2>13418-900, Piracicaba, SP</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>13418-900, Piracicaba, SP</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Boscariol Camargo, R L" sort="Boscariol Camargo, R L" uniqKey="Boscariol Camargo R" first="R. L." last="Boscariol-Camargo">R. L. Boscariol-Camargo</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratório de Biotecnologia Vegetal, CENA/USP</s1>
<s2>13400-970 Piracicaba, SP</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>13400-970 Piracicaba, SP</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cruz, R B" sort="Cruz, R B" uniqKey="Cruz R" first="R. B." last="Cruz">R. B. Cruz</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratório de Biotecnologia Vegetal, CENA/USP</s1>
<s2>13400-970 Piracicaba, SP</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>13400-970 Piracicaba, SP</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mourao Filho, F A A" sort="Mourao Filho, F A A" uniqKey="Mourao Filho F" first="F. A. A." last="Mourao Filho">F. A. A. Mourao Filho</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Dept. de Produção Vegetal, ESALQ/USP</s1>
<s2>13418-900, Piracicaba, SP</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>13418-900, Piracicaba, SP</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bergamin Filho, A" sort="Bergamin Filho, A" uniqKey="Bergamin Filho A" first="A." last="Bergamin Filho">A. Bergamin Filho</name>
<affiliation wicri:level="2"><inist:fA14 i1="03"><s1>Dept. de Entomologia Fitopatologia e Zoologia Agricola, ESALQ/USP</s1>
<s2>13418-900, Piracicaba, SP</s2>
<s3>BRA</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Brésil</country>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">10-0069435</idno>
<date when="2010">2010</date>
<idno type="stanalyst">PASCAL 10-0069435 INIST</idno>
<idno type="RBID">Pascal:10-0069435</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000272</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000728</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000213</idno>
<idno type="wicri:doubleKey">0032-0862:2010:Mendes B:reduction:in:susceptibility</idno>
<idno type="wicri:Area/Main/Merge">001C97</idno>
<idno type="wicri:Area/Main/Curation">001C59</idno>
<idno type="wicri:Area/Main/Exploration">001C59</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Reduction in susceptibility to Xanthomonas axonopodis pv. citri in transgenic Citrus sinensis expressing the rice Xa21 gene</title>
<author><name sortKey="Mendes, B M J" sort="Mendes, B M J" uniqKey="Mendes B" first="B. M. J." last="Mendes">B. M. J. Mendes</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratório de Biotecnologia Vegetal, CENA/USP</s1>
<s2>13400-970 Piracicaba, SP</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>13400-970 Piracicaba, SP</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cardoso, S C" sort="Cardoso, S C" uniqKey="Cardoso S" first="S. C." last="Cardoso">S. C. Cardoso</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Dept. de Produção Vegetal, ESALQ/USP</s1>
<s2>13418-900, Piracicaba, SP</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>13418-900, Piracicaba, SP</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Boscariol Camargo, R L" sort="Boscariol Camargo, R L" uniqKey="Boscariol Camargo R" first="R. L." last="Boscariol-Camargo">R. L. Boscariol-Camargo</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratório de Biotecnologia Vegetal, CENA/USP</s1>
<s2>13400-970 Piracicaba, SP</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>13400-970 Piracicaba, SP</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cruz, R B" sort="Cruz, R B" uniqKey="Cruz R" first="R. B." last="Cruz">R. B. Cruz</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratório de Biotecnologia Vegetal, CENA/USP</s1>
<s2>13400-970 Piracicaba, SP</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>13400-970 Piracicaba, SP</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mourao Filho, F A A" sort="Mourao Filho, F A A" uniqKey="Mourao Filho F" first="F. A. A." last="Mourao Filho">F. A. A. Mourao Filho</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Dept. de Produção Vegetal, ESALQ/USP</s1>
<s2>13418-900, Piracicaba, SP</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>13418-900, Piracicaba, SP</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bergamin Filho, A" sort="Bergamin Filho, A" uniqKey="Bergamin Filho A" first="A." last="Bergamin Filho">A. Bergamin Filho</name>
<affiliation wicri:level="2"><inist:fA14 i1="03"><s1>Dept. de Entomologia Fitopatologia e Zoologia Agricola, ESALQ/USP</s1>
<s2>13418-900, Piracicaba, SP</s2>
<s3>BRA</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Brésil</country>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Plant pathology</title>
<title level="j" type="abbreviated">Plant pathol.</title>
<idno type="ISSN">0032-0862</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Plant pathology</title>
<title level="j" type="abbreviated">Plant pathol.</title>
<idno type="ISSN">0032-0862</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteriosis</term>
<term>Citrus fruit</term>
<term>Citrus sinensis</term>
<term>Gene</term>
<term>Genetically modified organism</term>
<term>Oryza</term>
<term>Plant cankers</term>
<term>Plant pathology</term>
<term>Reduction</term>
<term>Rutaceae</term>
<term>Sensitivity</term>
<term>Xanthomonas axonopodis</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Réduction</term>
<term>Sensibilité</term>
<term>Bactériose</term>
<term>Gène</term>
<term>Phytopathologie</term>
<term>Rutaceae</term>
<term>Xanthomonas axonopodis</term>
<term>Organisme génétiquement modifié</term>
<term>Citrus sinensis</term>
<term>Oryza</term>
<term>Agrume</term>
<term>Chancre plante</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Organisme génétiquement modifié</term>
<term>Agrume</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">This work reports the evaluation of citrus canker resistance in transgenic lines of four sweet orange cultivars Hamlin, Natal, Pera and Valencia expressing the rice Xa21 R-gene. The Agrobacterium-mediated genetic transformation was performed and transgene integration was confirmed by Southern blot analyses. These analyses showed that the plants had undergone 1-3 independent transgene integration events. The genetic transformation efficiency was 18.6% for Hamlin, 8.3% for Natal, 3% for Pera and 11.3% for Valencia sweet orange. Transgene transcription was confirmed by northern blot analyses in most transgenic lines analysed. Transgenic lines of each of the cultivars were selected and propagated for resistance evaluation to Xanthomonas axonopodis pv. citri. Transgenic lines with a higher resistance to citrus canker were identified for cvs Hamlin, Natal and Pera. The lowest diseased leaf area values were detected for transgenic lines of Natal and Pera, cultivars which are considered moderately resistant to citrus canker in the field, indicating an influence of cultivar genetic background on the effectiveness of the Xa21 gene in controlling the pathogen. Transgenic lines in which transgene transcription was not detected did not show any improvement in resistance to the pathogen. These results show the integration and expression of the rice Xa21 gene in citrus and the possibility of using R-genes in controlling diseases in a heterologous genetic background.</div>
</front>
</TEI>
<affiliations><list><country><li>Brésil</li>
<li>États-Unis</li>
</country>
<region><li>État de São Paulo</li>
</region>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Mendes, B M J" sort="Mendes, B M J" uniqKey="Mendes B" first="B. M. J." last="Mendes">B. M. J. Mendes</name>
</noRegion>
<name sortKey="Boscariol Camargo, R L" sort="Boscariol Camargo, R L" uniqKey="Boscariol Camargo R" first="R. L." last="Boscariol-Camargo">R. L. Boscariol-Camargo</name>
<name sortKey="Cardoso, S C" sort="Cardoso, S C" uniqKey="Cardoso S" first="S. C." last="Cardoso">S. C. Cardoso</name>
<name sortKey="Cruz, R B" sort="Cruz, R B" uniqKey="Cruz R" first="R. B." last="Cruz">R. B. Cruz</name>
<name sortKey="Mourao Filho, F A A" sort="Mourao Filho, F A A" uniqKey="Mourao Filho F" first="F. A. A." last="Mourao Filho">F. A. A. Mourao Filho</name>
</country>
<country name="Brésil"><region name="État de São Paulo"><name sortKey="Bergamin Filho, A" sort="Bergamin Filho, A" uniqKey="Bergamin Filho A" first="A." last="Bergamin Filho">A. Bergamin Filho</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C59 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001C59 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:10-0069435
   |texte=   Reduction in susceptibility to Xanthomonas axonopodis pv. citri in transgenic Citrus sinensis expressing the rice Xa21 gene
}}

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'oranger

Reduction in susceptibility to Xanthomonas axonopodis pv. citri in transgenic Citrus sinensis expressing the rice Xa21 gene

Reduction in susceptibility to Xanthomonas axonopodis pv. citri in transgenic Citrus sinensis expressing the rice Xa21 gene

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri